Hoechst 33342 toxicity

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August undefined, 2024

NettetToxicity and safety [ edit] Because Hoechst stains bind to DNA, they interfere with DNA replication during cell division. Consequently, they are potentially mutagenic and … Nettet15. des. 2024 · Labeling Nuclear DNA with Hoechst 33342 [1] Step 1, Dilute the Hoechst stock solution 1:100 in H 2 O for use in labeling. Step 2, Aspirate the cell medium from …

Comparison of the supravital DNA dyes Hoechst 33342 and DAPI …

NettetToxicity Toxic, harmful Health Hazards Wear PPE at all times, handle with glove. Potential Hazards CNS and Liver; harmful by skin absorption, irritant mutagen. Carcinogenicity: No significant acute toxicological data identified AnaSpec Inc. 34801 Campus Drive, Fremont, CA 94555 Toll-Free: 800-452-5530 • Tel: 510-791-9560 • Fax: … Nettetfor 1 dag siden · Chlorpromazine, nystatin, and wortmannin solution were added to fibroblasts and incubated for 30 min followed by CSRBnps and incubated. At 30 min, 1, 4, and 12h exposure time, the CSRBnps were removed, and cells stained with Hoechst 33342 (NucBlue™ Live ReadyProbes™ Reagent, Invitrogen, USA)) in phenol red free … harvest chivas plumbing \u0026 electrical services

Phototoxicity of Hoechst 33342 in time-lapse fluorescence …

Nettet30. jun. 2016 · In the current study, we discovered that Hoechst 33342 (H342) could produce significant toxicity to the human neuroblastoma SK-N-SH cells at … NettetHoechst 33342 Page 1 of 2 PSF006 Hoechst 33342 Catalog number Product MW Size 40046 Hoechst 33342, 10 mg/mL in H 2 O 561.93 10 mL 40047 Hoechst 33342, trihydrochloride trihydrate 615.98 100 mg Storage and Handling Store Hoechst 33342, 10 mg/mL in H 2 O at 4°C, protected from light. Store Hoechst 33342, trihydrochloride … Nettet1. jan. 2009 · Hoechst 33342 is moderately water-soluble (up to 2% solutions can be prepared) and relatively non-toxic. The difference between the two bisbenzimides, Hoechst 33342 and 33258, is that 33342 has an ethyl group instead of a … harvest chocolate tecumseh mi

Cell cycle specific toxicity of the Hoechst 33342 stain in ... - PubMed

Hoechst 33342 for 72h live imaging, is it ok - ResearchGate

NettetIn experiments on three leukemic bone marrows, H33342 was shown to be more toxic than DAPI in terms of colony-forming capability. Although there is considerable … Nettet1. okt. 2010 · In this study we demonstrate that the common live-cell DNA stain Hoechst 33342 can cause apoptosis under CTLM conditions. Although toxicity is evident at … harvest chives without killing plantNettet28. feb. 2024 · Hoechst 33342 staining also showed obvious morphological characteristics of apoptosis in T24 cells incubated with Au–Ag@PDA NPs at 3 and 24 hours after laser irradiation. In particular, the apoptotic cells stained with Hoechst 33342 presented brighter blue fluorescence due to karyopyknosis and chromatin condensation in the … harvest choice weekly ad

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Hoechst 33342 toxicity

Nettet18. nov. 2024 · Hoechst 33342 (referred to as Hoechst) is a bis-benzamide dye that is relatively non-toxic and cell-permeable, making it suitable for a wide range of … Nettet1. aug. 1986 · The inherent toxicity of Hoechst 33342 and in particular its preferential toxicity to treated cells poses a significant limita tion on the use of this dye in …

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NettetIt is quite toxic to some cell types and potentiates may toxins bacuse it shares xenobiotic drug pumps. The BEST way to use Hoechst33342 dye is to make up a stock in DMSO … Nettet1. jan. 2009 · Hoechst 33342 is moderately water-soluble (up to 2% solutions can be prepared) and relatively non-toxic. The difference between the two bisbenzimides, Hoechst 33342 and Cellular toxicity of Hoechst 33342 Cell types differ with respect to their cytotoxic and kinetic responses to Hoechst 33342.

NettetFeatures of Hoechst 33342 Fluorescent Stain: • Hoechst dye—blue fluorescent stain specific for DNA (i.e., nuclei of eukaryotic cells) • Convenient—provided as an easy-to … NettetHoechst 33342 induced reactive oxygen species and impaired expression of cytochrome c oxidase subunit 1 leading to cell death in irradiated human cancer cells Oxidative stress …

NettetBisbenzimide ( Hoechst 33342) is an organic compound used as a fluorescent stain for DNA in molecular biology applications. [1] Several related chemical compounds are used for similar purposes and are … NettetCytotoxicity, Mutagenicity and DNA damage by Hoechst 33342. Hoechst 33342 can be used for flow microfluorimetry (FMF) analysis of the DNA content of living Chinese …

NettetHoechst 33342染色常用于细胞凋亡检测，染色后用荧光显微镜观察或流式细胞仪检测。 Hoechst 33342也常用于普通的细胞核染色，或常规的DNA染色。 Hoechst 33342的最大激发波长为346nm，最大发射波长为460nm；Hoechst 33342和双链DNA结合后，最大激发波长为350nm，最大发射波长为461nm。

NettetIn 1978, another DNA-binding fluorochrome, Hoechst 33342, was reported as a live stain of cells suitable for subsequent cell sorting. ... (LC50), an essential parameter in toxicology, is often determined for a specific time. Here, we calculated the LC50 after 72 … harvest christian academy calendarNettet20. feb. 2015 · The chapter also includes references on the use, properties, and safety/toxicity of Hoechst 33342 on humans, animals and the environment. It presents … harvest choice market weekly adNettet12. apr. 2024 · Nuclei were stained with Hoechst 33342 (1/1000). Anti-endomucin antibody (sc-65495, Santa Cruz, diluted 1/200) was used to stain vessels in bone marrow and spleen. harvest choice market anaheim katellaNettet15. sep. 2024 · hoechst 33342染色步骤. 1. 将荧光染料（Hoechst 33342、AO、PI 或 EB）以合适浓度直接添加到细胞培养基中。. 2. 将细胞孵育 5 分钟。. 3. 在倒置荧光显微镜下直接观察细胞。. 如果需要，在对转染细胞进行重要染色后修复细胞，以进一步详细分析形态：. 1.用PBS清洗细胞 ... harvest child enrichment centerNettetDisplaying low-cytotoxic effects and increased cell permeability, Hoechst stains are ideal for live- and fixed-cell fluorescent DNA staining and nuclei imaging techniques, such as cell counting, automated DNA determination, cell cycle analysis, chromosome sorting, and nuclear counterstaining. harvest christian academy daycareNettetPage 2/6 Safety Data Sheet acc. to OSHA HCS Printing date 01/14/2024 Revision date 01/14/2024 Trade name: Hoechst 33342 (hydrochloride) (Contd. from page 1) harvest christian academy edinburg calendarNettetI'm planning to stain fixed Vero nuclei with Hoechst 33342. The protocol manual provides quite a range for both concentration (0.2 - 2 µg/ml) and incubation time (1 - 15 min) when staining... harvest christian academy delaware